Bilaney Consultants, basé en Allemagne et au Royaume-Uni, fournit des équipements et des logiciels de recherche de qualité aux chercheurs en sciences de la vie et aux chercheurs biomédicaux en Europe depuis 1966. N'hésitez pas à nous contacter si vous avez des questions sur le produit ou si vous avez besoin d'assistance.

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Standard Infuse/Withdraw PHD ULTRA™ 4400 Programmable Syringe Pump

SKU HBI-IW-70-3010
In stock
Product Details

The PHD ULTRA™ 4400 is the solution for your most demanding fluidics applications. This pump represents the latest technology in syringe pumps and was developed utilizing the feedback of the world’s largest populations of syringe pump users.

Item# Description Pricing
70-3010 PHD ULTRA™ 4400 Syringe Pump Infuse/Withdraw Programmable Get a Quote
70-3030 PHD ULTRA™ RS-232 RJ-11 Connectors Option (If needed, must be purchased at the same time as the Ultra Pump) Get a Quote
70-3033 PHD ULTRA Analog Control Input Option (If needed, must be purchased at the same time as the PHD ULTRA Programmable Pump) Get a Quote
70-3034 PHD ULTRA Internal Fan Option (Required if external operating ambient is >35°C. If needed, fan must be purchased at the same time as the PHD ULTRA™ Pump) Get a Quote


The PHD ULTRA™ 4400 will change the way you think about syringe pumps. There are three major areas which make the PHD ULTRA™ 4400 the new standard for syringe pumps:

  • Superior mechanical drive mechanism and syringe holding mechanics to achieve the highest performance of any syringe pump
  • EZ PRO Software and user interface allow easy programming of methods from simple to complex, all without the use of a PC
    • Preprogrammed methods for simple to complex operations that allow you to be up and running with the push of a button
    • LCD, high resolution color touch screen for powerful functionality, yet easy to use
  • Multiple levels of versatility
    • Configurations: standard and remote
    • Connectivity: RS-232 and USB for PC Control; RS-485 for daisy chaining

Full Description

The PHD ULTRA™ 4400 is the solution for your most demanding fluidics applications. This pump represents the latest technology in syringe pumps and was developed utilizing the feedback of the world’s largest populations of syringe pump users.


  • Superior drive mechanism for unmatched smooth flow, accuracy and precision
  • From picoliter to 216 ml/min flow rates
  • Advanced programming for true multi methods without a PC
  • Quick start methods
  • Alpha/numeric keyboard without a PC
  • Real and relative time clocks
  • Icon operation
  • New color LCD touch screen
  • Up-front control knobs for ease of operation
  • Vertical or horizontal orientation
  • Adjustable linear force to 200 lbs across the entire flow range
  • Daisy chain
  • Remote configuration placement
  • CE, ETL (UL, CSA), WEEE, EU RoHS, & CB Scheme
  • 2-year warranty


  • Nanofluidics
  • Drug/Nutritional infusions
  • Electrospinning
  • Reaction chamber addition
  • Viscous Solutions
  • High Pressure Injection
  • Highly Corrosive Fluids
  • Fluid Blending
  • Flow programming
  • Gradients
  • % composition step changes
  • Large flow deliveries
  • I/O interactive experiments

The PHD ULTRA™ 4400 will change the way you think about syringe pumps. There are three major areas which make the PHD ULTRA the new standard for syringe pumps:

1. Superior pending mechanical drive mechanism and syringe holding mechanics to achieve the highest performance of any syringe pump

2. EZ PRO Software and user interface allow easy programming of methods from simple to complex, all without the use of a PC
– Preprogrammed methods for simple to complex operations that allow you to be up and running with the push of a button
– LCD, high resolution color touch screen for powerful functionality, yet easy to use

3. Multiple levels of versatility
a. Configurations: standard and remote
b. Connectivity: RS-232 and USB for PC Control; RS-485 for daisy chaining

Harvard Apparatus introduces the next generation of syringe pumps, the PHD ULTRA™ 4400 for the most demanding applications.

Since 1901 Harvard Apparatus has been supporting bioresearch fluidics requirements with the culmination being the introduction of the first commercial syringe pump for bioresearch in 1956. Since 1956, over 70,000 satisfied syringe pump users around the world have made Harvard Apparatus syringe pumps the worlds #1 choice.

The PHD ULTRA™ Syringe Pump series is a family of high-accuracy, microliter- and milliliter-compatible pumps designed for versatile technical use including mass spectroscopy, calibration, drug and nutritional infusions, microdialysis, dispensing, chromatography and LC/HPLC.

Highest Accuracy and Precision
The PHD ULTRA™ 4400 syringe pump family has a new patent pending fluidics drive mechanism which assures ease of use and high performance, for smoother, more accurate flow rates than any other syringe pump. Flow rates are accurate within 0.35% and reproducibility within 0.05%. A microprocessor-controlled, small step angle stepping motor drives a lead screw and pusher block. Advanced micro-stepping techniques are employed to further reduce the step angle to eliminate flow pulsation.

Advanced Programming Features:
• Flow Programming – Change the flow with time, volume or a triggered event as many times as you like
• Bolus – Inject a drug (or drugs) in a high quantity at once. The bolus injection can be made in time or volume.
• Concentration Delivery – Calibrate flow in concentration units of mg/kg easily so flow is calibrated to concentration of drug and animal weight.
• Gradients
• % ratio – up to three solvents
• I/O — dedicated and user defined I/O
• Pulsed Flow- so you can program the pulse easily

Widest Flow Rate Range
This pump is engineered to provide flow accuracy within 0.35% and reproducibility within 0.05%. A Single syringe from 0.5 µl to 140 ml pumps at a range of 3.06 pl/min to 216 ml/min.

Maximum Experimental Versatility
The PHD ULTRA™ 4400 features true Multi-Pump Operation. The pump can be oriented vertically or horizontally for optimum experimental connectivity. This pump comes standard to hold 1 syringe.

Easy-to-Use Interface
The PHD ULTRA™ 4400; color LCD touch screen graphic interface is divided into three basic areas: Operations Display, Message Area, and Navigation. This configuration allows you to easily move through all menu selections and data entry by gently touching the onscreen buttons with a finger or the tip of a soft, non-sharp object such as a pencil eraser.

The Methods Main or Quick Start screens are the primary home for the applications. From those screens you access all the commands needed to operate the PHD ULTRA™ 4400 as well as the main system settings.

The Message Area of the touch screen is used to display helpful instructions for the currently displayed screen. It is also used to display error or warning messages to indicate problem conditions in a Method or error conditions during pump operation.

The software is organized into three main Navigational branches, the quick start operations, preloaded/user-defined Methods, and systems settings. You can control operations directly with the touch screen or remotely from an independent computer or device via the external I/O interface.

Program Description
To operate the PHD ULTRA™ 4400, the user defines all the required parameters for infusing or withdrawing liquids through a Pump Control Method. The basic operation is a simple 4-step procedure:

1. Select a method
2. Enter operating parameters
3. Preview your method
4. Run your method

• Animal Infusions or Withdrawals – The PHD ULTRA™ 4400 will control the delivery of varying % of nutrients or drugs infused into animals, flush lines using catheters, needles, cannulae or microdialysis.
• Proportioning and Delivering of Mixtures – Mixing gradients or proportions with independent control of two liquids.
• Aerosol for Coating – The pump at high pressure can create an aerosol for the delivery of coating materials such as pharmaceutical tablets and aerosol studies.
• Delivery to Mass Spectroscopy – The delivery of fluids to the MS for calibration, matrix addition or ESI sample.
• Compensating Flows – The continuous infusion and simultaneous withdrawal of liquids for cell cultures or perfusion chambers.
• Dispensers/Injectors — Adhesives, Cell injection, MRI Dyes, Activators/Enzymes, Flow injection, Microreaction vessels, or Stereotaxic delivery.

Advanced GLP Documentation Features:
• Experiment parameter download information to PC
• Alpha/numeric capability

Pump Models
The PHD ULTRA™ 4400 Syringe Pump is available as an Infuse/Withdraw Programmable model. This model supports both infusion and withdrawal operations and can use both simplified pumping profiles or the more advanced pump profiles and I/O settings that permit interactions with external devices. In addition to supporting all of the advanced programming tools available on the PHD ULTRA™ 4400, this model also allows users to create and store multiple user-defined methods on the pump.

A full range of accessories are compatible with the PHD ULTRA™ 4400 including syringe heaters, connectors, tubing, syringes and more.


Specifications 70-3010
Accuracy ±0.35%
Classification Class I
Dimensions L x D x H in (cm) 12 x 8.5 x 7.25 (30.48 x 21.59 x 18.4)
Dimensions LxDxH in (cm) N/A
Display 4.3″ WQVGA TFT Color Display with Touch
Drive Motor 1.8° Stepper Motor
Environmental Humidity 20% to 80% RH, non condensing
Environmental Operating Temperature English 40°F to 104°F
Environmental Operating Temperature Metric 4°C to 40°C
Environmental Storage Temperature English 14°F to 158°F
Environmental Storage Temperature Metric -10°C to 70°C
Flow Rate Maximum 216 ml/min using a 140 ml syringe
Flow Rate Minimum 3.06 pl/min using a 0.5 µl syringe
I O TTL Connectors 15 pin D-Sub Connector
Input Power 50 W, 0.5 A fuse
Installation Category II
Max Linear Force 200 lbs @ 100% Force Selection
Mode of Operation Continuous
Motor Drive Control Microprocessor with 1/16 microstepping
Net Weight English 12.1 lbs
Net Weight Metric 5.5 kg
No of Syringes 1
Non Volatile Memory Storage of all settings
Number of Microsteps per one rev of Lead Screw 6,400
Pollution Degree 1
Pump Configuration Standard
Pump Function Infuse/Withdraw, Programmable
Pusher Travel Rate Maximum 190.8 mm/min
Pusher Travel Rate Minimum 0.36 µm/min
RS dash 232 Connectors 9 pin D-Sub Connector
Rack Type Standard Rack
Regulatory Certifications CE, ETL (UL, CSA), WEEE, EU RoHS and CB Scheme
Step Rate Maximum 26 µsec/µstep
Step Rate Minimum 27.5 sec/µstep
Syringe Size Maximum 140 ml
Syringe Size Minimum 0.5 µl
USB Connectors Type B
Voltage Range 100–240 VAC, 50/60 Hz
RS – 232 Connectors IEEE-1394, 6pos

Journal Articles

Xizhong Cui, PhD; Yvonne Fitz, BS; Yan Li, MD; Ping Qiu, Ph.D; Steve Solomon, Ph D; Mariam Al-Hamad, BS & Peter Q. Eichacker, MD (2013 ) Pilot Investigation Of A Multi-Channel Automated Drug Delivery System For Blood Pressure Regulated Vasopressor Administration In A Rat Model ATS Journals

Amber L. Alhadeff , Matthew R. Hayes , Harvey J. Grill (2014 ) Leptin receptor signaling in the lateral parabrachial nucleus contributes to the control of food intake American Journal of Physiology

Vivek Sharma, Simon J. Haward, James Serdy, Bavand Keshavarz, Asa Soderlund, Phil Threlfall-Holmes & Gareth H. McKinley (2015 ) The rheology of aqueous solutions of ethyl hydroxy-ethyl cellulose (EHEC) and its hydrophobically modified analogue (hmEHEC): extensional flow response in capillary break-up, jetti Royal Society of Chemistry

Amber L. Alhadeff, Laura E. Rupprecht, and Matthew R. Hayes (2011 ) GLP-1 Neurons in the Nucleus of the Solitary Tract Project Directly to the Ventral Tegmental Area and Nucleus Accumbens to Control for Food Intake Endocrine Society

Ryan W. Mutharda & Scott L. Diamond (2013 ) Side view thrombosis microfluidic device with controllable wall shear rate and transthrombus pressure gradient Lab On A Chip

G. L. Scaglione, S. Lancellotti1, M. Papi1, M. De Spirito, A. Maiorana, L. Baronciani, M. T. Pagliari, A. Arcovito, E. Di Stasio, F. Peyvandi, R. De Cristofaro (2013 ) The type 2B p.R1306W natural mutation of von Willebrand factor dramatically enhances the multimer sensitivity to shear stress The Journal of Thrombosis and Haemostasis

Youri Gendelb, Oana Davidb & Matthias Wesslinga (2013 ) Microtubes made of carbon nanotubes Science Direct

Jidong Wang, Wenwen Chen, Jiashu Sun, Chao Liu, Qifang Yin, Lu Zhang, Yunlei Xianyu, Xinghua Shi, Guoqing Hu & Xingyu Jiang (2014 ) A microfluidic tubing method and its application for controlled synthesis of polymeric nanoparticles Lab On A Chip

J. D. Welsh, T. V. Colace, R. W. Muthard, T. J. Stalker, L. F. Brass & S. L. Diamond (2012 ) Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse The Journal of Thrombosis and Haemostasis

Dominika Ogończyk, Mateusz Gocyla, Marcin Opallo (2014 ) Electrochemical response of catalytic nanoparticles in Flow Injection Analysis system Science Direct

James O. Hardin, Thomas J. Ober, Alexander D. Valentine & Jennifer A. Lewis (2015 ) Microfluidic Printheads for Multimaterial 3D Printing of Viscoelastic Inks Advanced Materials

Nan Li, Miguel F. Diaz, Pamela L. Wenzel Ph.D. (2014 ) Application of Fluid Mechanical Force to Embryonic Sources of Hemogenic Endothelium and Hematopoietic Stem Cells Methods in Molecular Biology

Wahyudionoa, Kanako Murakamia, Siti Machmudahb, Mitsuru Sasakia & Motonobu Gotob (2011 ) Production of nanofibers by electrospinning under pressurized CO2 High Pressure Research: An International Journal

Iulia – Rodica Damian, Nicoleta Octavia Tănase, Ștefan – Mugur Simionescu, Mona Mihăilescu (2015 ) Vortex Rings – Experiments and Numerical Simulations Mathematical Modelling in Civil Engineering

C. Liua, J.D. Yeagera & K.J. Ramosa (2015 ) Bonding energy of Sylgard on fused quartz: an experimental investigation Philosophical Magazine

Stephen G. Newman , Kyoungmi Lee , Jianghuai Cai , Lu Yang , William H. Green , and Klavs F. Jensen (2014 ) Continuous Thermal Oxidation of Alkenes with Nitrous Oxide in a Packed Bed Reactor Industrial & Engineering Chemisrty Research

Jinyoung Baekm Dr. Peter M. Allen, Prof. Moungi G. Bawendi & Prof. Klavs F. Jensen (2010 ) Investigation of Indium Phosphide Nanocrystal Synthesis Using a High-Temperature and High-Pressure Continuous Flow Microreactor Angwandte Chemie

I. R. G. Ogilvie, V. J. Siebe, M. C. Mowlem, and H. Morgan (2011 ) Temporal Optimization of Microfluidic Colorimetric Sensors by Use of Multiplexed Stop-Flow Architecture Analytical Chemistry

Isabella Pallotta, Ph.D., Michael Lovett, Ph.D., David L. Kaplan, Ph.D. & Alessandra Balduini, M.D. (2011 ) Three-Dimensional System for the In Vitro Study of Megakaryocytes and Functional Platelet Production Using Silk-Based Vascular Tubes Tissue Engineering

Laurent Pellegatti and Stephen L. Buchwald (2012 ) Continuous-Flow Preparation and Use of β-Chloro Enals Using the Vilsmeier Reagent Organic Process Research & Development

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